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The primers should hybridize a region without any known SNP (use appropriate SNP databases) and the subsequent PCR amplicon could include more than one SNP. Primers were designed to flank the coding regions, without second structure (hairpin or dimer formation) and to be annealed at 60C using Primer Express software (Applied Biosystems, Foster City, CA). All primers should be HPLC ...

Cheap Trick’s performance at the upcoming Hall of Fame ceremony is noteworthy because original drummer Bun E. Carlos will appear with Zander, Nielsen and bassist Tom Petersson onstage for the first time in six years. (Carlos and the band had a legal dispute following Carlos exit from Cheap Trick in 2010.) Current drummer Daxx Nielsen (Rick Nielsen son) will be ...

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